SIST-TS CEN ISO/TS 22115:2021 PDF

Animal and vegetable fats and oils - Separation of lipid classes by capillary gas chromatography (fingerprint method) (ISO/TS 22115:2021)

Название (английский):

SIST-TS CEN ISO/TS 22115:2021

Animal and vegetable fats and oils - Separation of lipid classes by capillary gas chromatography (fingerprint method) (ISO/TS 22115:2021)

Статус документа:: Действующий
Формат:: Электронный (PDF)
Количество страниц:: 29
Дата публикации:: 17 августа 2021 г.
ICS:: 67.200.10
Технический комитет:: KŽP - Agricultural food products

Язык документа:

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Срок поставки:5 раб. дн. (перевод)

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Описание (английский):

This document specifies a method for the semi-quantitative analysis of oils, fats and oil/fat-related samples (deodistillates). It is applicable to the screening of oils, fats and oil/fat-related samples to obtain main (e.g. triglycerides) and minor (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol) component information in one single analysis. For a truly quantitative analysis of pre-identified compound classes, specific methods are more appropriate. The method can also be used as a useful qualitative screening tool for the relative comparison of sample compositions.

Технические детали

SKU: SIST-TS CEN ISO/TS 22115:2021

Abstract

Overview

CEN ISO/TS 22115:2021 - "Animal and vegetable fats and oils - Separation of lipid classes by capillary gas chromatography (fingerprint method)" - defines a semi-quantitative capillary GC fingerprinting method for oils, fats and oil/fat-related samples (including deodistillates). The technique provides a single-analysis overview of main lipid classes (e.g. triglycerides) and minor components (e.g. sterols, sterol esters, tocopherols, wax esters, fatty alcohols, glycerol). It is intended for screening and relative comparison of sample compositions rather than fully quantitative determination of pre‑identified compound classes.

Key topics and technical requirements

Analytical principle: Hydroxylated compounds are converted to silyl derivatives and analyzed by capillary gas chromatography (GC) on a high‑temperature column; non‑hydroxylated (apolar) compounds remain unaffected.
Instrumentation: Requires a GC for capillary columns with an on‑column injector (or equivalent), temperature‑programmable oven and flame ionization detector (FID); a fused silica capillary column rated up to ~400 °C with low film thickness is recommended.
Reagents and standards:
- Silylation reagent (example mixture: MSHFBA + methylimidazole, 1 ml + 50 µL).
- Internal standard: 1,2,3‑tridecanoylglycerol.
- Reference compounds used for response factor determination include oleic acid, mono/di/triglycerides, tocopherols, cholesterol, cholesterol esters, squalene, plant sterol mix, etc.
- Solvents: isooctane, chloroform (chromatographic grade).
Sample preparation: Follow ISO 661 for test‑sample preparation; perform silylation of sample and standards prior to GC.
Data treatment: Peak identification and integration, calculation of response factors from reference standards, and semi‑quantitative reporting. The method includes criteria for repeatability, reproducibility and interlaboratory precision (informative annexes with example chromatograms and interlab results).
Limitations: Designed as a method-use dedicated, validated quantitative methods when absolute quantification of a given class is required.

Abstract

Overview

Key topics and technical requirements

Analytical principle: Hydroxylated compounds are converted to silyl derivatives and analyzed by capillary gas chromatography (GC) on a high‑temperature column; non‑hydroxylated (apolar) compounds remain unaffected.
Instrumentation: Requires a GC for capillary columns with an on‑column injector (or equivalent), temperature‑programmable oven and flame ionization detector (FID); a fused silica capillary column rated up to ~400 °C with low film thickness is recommended.
Reagents and standards:
- Silylation reagent (example mixture: MSHFBA + methylimidazole, 1 ml + 50 µL).
- Internal standard: 1,2,3‑tridecanoylglycerol.
- Reference compounds used for response factor determination include oleic acid, mono/di/triglycerides, tocopherols, cholesterol, cholesterol esters, squalene, plant sterol mix, etc.
- Solvents: isooctane, chloroform (chromatographic grade).
Sample preparation: Follow ISO 661 for test‑sample preparation; perform silylation of sample and standards prior to GC.
Data treatment: Peak identification and integration, calculation of response factors from reference standards, and semi‑quantitative reporting. The method includes criteria for repeatability, reproducibility and interlaboratory precision (informative annexes with example chromatograms and interlab results).
Limitations: Designed as a method-use dedicated, validated quantitative methods when absolute quantification of a given class is required.

SIST-TS CEN ISO/TS 22115:2021 PDF

Технические детали

Abstract

Overview

Key topics and technical requirements

Abstract

Overview

Key topics and technical requirements

Applications and users

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